Recombinant human BCMA/TNFRSF17

Product Name

Recombinant Human B-Cell Maturation Antigen/TNFRSF17

(rHuBCMA/TNFRSF17)

Synonyms

TNFRSF17, BCM, CD269

Source

Expressed in E. coli.

Molecular weight

Approximately 5.4kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.

Accession

Q02223 Ala5- Ala54

AA Sequence

AGQCSQNEYFDSLLHACIPCQLRCSSNTPPLTCQRYCNASVTNSVKGTNA

Purity

＞98% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40ng/ml, corresponding to a specific activity of >2.5×10⁴ IU/mg in the presence of 100ng/ml human APRIL.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA.

Endotoxin level

＜0.1EU/μg rHuBCMA/TNFRSF17 protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored ≤-20℃. 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.