Recombinant murine HB-EGF

Product Name

Recombinant Murine Heparin-binding EGF-like Growth Factor

(rMuHB-EGF)

Synonyms

HBEGF, DT-R

Source

Expressed in E. coli. 

Molecular weight

Approximately 9.8kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

Accession

Q06186 Asp63-Leu148

AA Sequence

DLEGTDLNLFKVAFSSKPQGLATPSKERNGKKKKKGKGLGKKRDPCLRKYKDYCIHGECRYLQEFRTPSCKCLPGYHGHRCHGLTL

Purity

＞97% by SDS-PAGE or HPLC

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 10 mM PB, 500 mM NaCl, pH 7.4.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 ×10⁶U/mg.

Endotoxin level

＜0.01EU/μg rMuHB-EGF protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution.

12 months from date of receipt, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution.

3 months, -20 to -70℃ under sterile conditions after reconstitution.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.