Recombinant human CCL25

Product Name

Recombinant Human Thymus Expressed Chemokine/CCL25

(rHuTECK/CCL25)

Synonyms

TECK, CCL25, SCYA25, Ckb15

Source

Expressed in E. coli. 

Molecular weight

Approximately 14.3kDa, a single non-glycosylated polypeptide chain containing 128 amino acids.

Accession

O15444 Gln24-Leu150 with a N-terminal methionine

AA Sequence

MQGVFEDCCLAYHYPIGWAVLRRAWTYRIQEVSGSCNLPAAIFYLPKRHRKVCGNPKSREVQRAMKLLDARNKVFAKLHHNTQTFQAGPHAVKKLSSGNSKLSSSKFSNPISSSKRNVSLLISANSGL

Purity

＞97% by SDS-PAGE or HPLC

Formulation

Lyophilized from a 0.2mm filtered solution in 20mM PB, 150mM NaCl, pH7.4.

Biological activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 1.0-10.0ng/ml.

Endotoxin level

Less than 0.1EU/mg of rHuTECK/CCL25 as determined by LAL method

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution.

12 months from date of receipt, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution.

3 months, -20 to -70℃ under sterile conditions after reconstitution.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.