Recombinant human CCL1

Product Name

Recombinant human I-309/CCL1

(rHuI-309/CCL1)

Synonyms

Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

Source

Expressed in E. coli.

Molecular weight

Approximately 8.6kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.

Accession

P22362 Ser23-Lys96

AA Sequence

SKSMQVPFSRCCFSFAEQEIPLRAILCYRNTSSICSNEGLIFKLKRGKEACALDTVGWVQRHRKMLRHCPSKRK

Purity

＞98% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB,150 mM NaCl, pH 7.4.

Endotoxin level

＜0.1EU/μg rHuI-309/CCL1 protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored ≤-20℃. 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.