Recombinant human PDGF-BB

Product Name

Recombinant Human Platelet-derived Growth Factor-BB

（rHuPDGF-BB）

Source

Expressed in E. coli 

Molecular weight

Approximately 24.8kDa, containing two 110 amino acid residues polypeptide

Accession

P01127 Ser82-Thr190 with an N-terminal Methionine

AA sequence

MSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT

Purity

＞97% by SDS-PAGE or HPLC

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3x10⁵ IU/mg protein

Formulation

Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4

Endotoxin level

＜0.1EU/μg rHuPDGF-BB protein

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

3、 Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored ≤-20℃. 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.