Recombinant human GDNF

Product Name

Recombinant Human Glial Cell Line-derived Neurotrophic Factor

(rHuGDNF)

Synonyms

ATF, ATF-1

Source

Expressed in E. coli.

Molecular weight

Approximately 30.1kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

Accession

P39905 Ser77-Ile211

AA Sequence

SPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI

Purity

＞97% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 0.1ng/ml, corresponding to a specific activity of >5.0 × 10⁷IU/mg.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB,500mM NaCl, 0.05% Tween-20, pH7.4.

Endotoxin level

＜0.1EU/μg rHuGDNF protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃ 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.