Recombinant human LIF

Product Name

Recombinant Human Leukemia Inhibitory Factor 

(rHuLIF)

Source

Expressed in E. coli. 

Molecular weight

Approximately 19.7kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

Accession

P15018  Ser23-Phe202

AA Sequence

SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF

Purity

＞98% by SDS-PAGE or HPLC

Formulation

Lyophilized from a 0.2mm filtered solution in PBS, pH 7.2.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

Endotoxin level

Less than 0.1EU/mg of rHuLIF as determined by LAL method

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored≤-20℃.

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.