Recombinant human FGF-20

Product Name

Recombinant Human Fibroblast Growth Factor-20

(rHuFGF-20)

Synonyms

Fibroblast Growth Factor-20, FGFK

Source

Expressed in E. coli.

Molecular weight

Approximately 23.5kDa, a single non-glycosylated polypeptide chain containing 211 amino acids.

Accession

Q9NP95 Met1-Thr211

AA Sequence

MAPLAEVGGFLGGLEGLGQQVGSHFLLPPAGERPPLLGERRSAAERSARGGPGAAQLAHLHGILRRRQLYCRTGFHLQILPDGSVQGTRQDHSLFGILEFISVAVGLVSIRGVDSGLYLGMNDKGELYGSEKLTSECIFREQFEENWYNTYSSNIYKHGDTGRRYFVALNKDGTPRDGARSKRHQKFTHFLPRPVDPERVPELYKDLLMYT

Purity

＞96% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED₅₀ is ≤ 5ng/ml in the presence of 1µg/ml heparin, corresponding to a specific activity of ≥ 2 x 10⁵ units/mg.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 50mMTris-HCL pH7.6, with NaCL and tween-20 as protectant.

Endotoxin level

＜0.01EU/μg rHuFGF-20 protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃ 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.