Recombinant human IL-5

Product Name

Recombinant Human Interleukin-5

(rHuIL-5)

Synonyms

B-cell differentiation factor I, Eosinophil differentiation factor, TRF

Source

Expressed in E. coli.

Molecular weight

Approximately 26.5kDa, a disulfide-linked homodimeric protein containing two 116 amino acids.

Accession

P05113 Ile20-Ser134 with a N-terminal Met

AA Sequence

MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

Purity

＞98% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 0.1ng/ml, corresponding to a specific activity of > 1.0 ×10⁷ IU/mg.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB, pH7.5, 3% trehalose, 0.1% Tween-20.

Endotoxin level

＜0.01EU/μg rHuIL-5 protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored ≤-20℃. 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.