Recombinant human IL-1β

Product Name

Recombinant Human Interleukin-1 beta

(rHuIL-1β)

Synonyms

Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF, Endogenous Pyrogen, EP

Source

Expressed in E. coli.

Molecular weight

Approximately 17.3kDa, a single non-glycosylated polypeptide chain containing153 amino acids.

Accession

P01584 Ala117-Ser269

AA Sequence

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

Purity

＞97% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 1.0pg/ml, corresponding to a specific activity of > 1.0 × 10⁹ IU/mg.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Endotoxin level

＜0.01EU/μg rHuIL-1βprotein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in a sterile aqueous buffer to an appropriate concentration. 

Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃ 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8 ℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.