Recombinant murine KGF-1/FGF-7

Product Name

Recombinant Murine Keratinocyte Growth Factor 1/FGF-7

(rMuKGF-1/FGF-7)

Synonyms

HBGF-7

Source

Expressed in E. coli.

Molecular weight

Approximately 18.7kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

Accession

P36363 Cys32-Thr194

AA Sequence

CNDMSPEQTATSVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNSYNIMEIRTVAVGIVAIKGVESEYYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHSGGEMFVALNQKGIPVKGKKTKKEQKTAHFLPMAIT

Purity

＞96% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ IU/mg.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, 800mM NaCl, pH 8.0.

Endotoxin level

＜0.01EU/μg rMuKGF-1/FGF-7 protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled PBS or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.5mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃.

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.