Recombinant human SCD40-L

Product Name

Recombinant Human soluble CD40 Ligand

(rHusCD40L)

Synonyms

soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM

Source

Expressed in E. coli. 

Molecular weight

Approximately 16.3kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.

Accession

P29965 Met113-Leu261

AA Sequence

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Purity

＞97% by SDS-PAGE or HPLC

Formulation

Lyophilized from a 0.2mm filtered solution in in 20mM PB, 150mM NaCl, pH 7.0.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.

Endotoxin level

Less than 1 EU/mg of rHusCD40L as determined by LAL method

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. 

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution.

12 months from date of receipt, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution.

3 months, -20 to -70℃ under sterile conditions after reconstitution.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.