Recombinant human IFN-α1b

Product Name

Recombinant Human Interferon-alpha1b

(rHuIFN-α1b)

Cat. No.

GH029

Source

Expressed in E. coli.

Molecular weight

Approximately 19.5kDa, a single non-glycosylated polypeptide chain containing 167 amino acids

Accession

P01562 Cys24-Glu189 with an N-terminal Met(A115V)

Protein amino acid sequence

MCDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNVDSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE

Purity

＞98% by SDS-PAGE or HPLC

Formulation

Lyophilized from a 0.2μm filtered solution in 20mMPB,300mMNaCl, 5%trehalose 0.01%tween-80 1mMDTT.

Biological activity

Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 ×10⁸ IU/mg.

Endotoxin level

＜0.1EU/μg rHuIFN-α1b protein

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in a sterile aqueous buffer to an appropriate concentration. 

Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃.

36 months, -20 to -70℃ as supplied;

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.