Thermal stable Recombinant human bFGF

Product Name

Recombinant Human Basic Fibroblast Growth Factor mutant G3

(bFGF-G3)

Synonyms

Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

Source

Expressed in E. coli.

Molecular weight

Approximately 17.3kDa, a single non-glycosylated polypeptide chain containing155 amino acids.

Accession

P09038 Ala135-Ser288 with an N-terminal methionine and several site mutations

AA Sequence

MAAGSITTLPALPEDGGSGAFPPGHFKDPKLLYCKNGGFFLRIHPDGRVDGTRDKSDPFIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLYAIKNVTDECFFFERLEENNYNTYRSRKYPSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

Purity

＞97% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0 × 10⁷ IU/mg.

Formulation

Lyophilized from 20mMTris,150mM NaCl, 5%trehalose,0.2‰Tween-20,pH7.6.

Endotoxin level

＜0.01EU/μg rHubFGF-G3 protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

3、 Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored ≤-20℃.

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.