Recombinant human TGFα

Product Name

Recombinant Human transforming Growth Factor alpha

(rHuTGFα)

Synonyms

Transforming Growth Factor-α, Sacroma growth factor, TGF-type I, ETGF

Source

Expressed in E. coli. 

Molecular weight

Approximately 5.7kDa, a single non-glycosylated polypeptide chain containing 51 amino acids.

Accession

P01135 Val40-Ala89 with an N-terminal methionine

AA Sequence

MVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA

Purity

＞96% by SDS-PAGE or HPLC

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, with 0.02% Tween-20, pH 7.4.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤0.2ng/ml, corresponding to a specific activity of≥5 x 10⁶ units/mg

Endotoxin level

＜0.01EU/μg rHuTGFα protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution.

12 months from date of receipt, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution.

3 months, -20 to -70℃ under sterile conditions after reconstitution.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.