Recombinant human TSLP

Product Name

Recombinant Human Thymic stromal lymphopoietin

(rHuTSLP)

Synonyms

thymic stromal lymphopoietin; TSLP

Source

Expressed in E. coli.

Molecular weight

Approximately 15.1kDa, a single non-glycosylated polypeptide chain containing 132 amino acids.

Accession

Q969D9 Tyr29-Gln159, with an N-terminal Met

AA Sequence

MYDFTNCDFEKIKAAYLSTISKDLITYMSGTKSTEFNNTVSCSNRPHCLTEIQSLTFNPTAGCASLAKEMFAMKTKAALAIWCPGYSETQINATQAMKKRRKRKVTTNKCLEQVSQLQGLWRRFNRPLLKQQ

Purity

＞98% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3ng/ml, corresponding to a specific activity of > 3.3×10⁶IU/mg.

Formulation

Lyophilized from a 0.2µm filtered solution in 20mM PB, 150mM NaCl, pH7.4.

Endotoxin level

＜0.1EU/μg rHuTSLP protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored ≤-20℃. 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.