Recombinant human IL-11

Product Name

Recombinant Human Interleukin-11

(rHuIL-11)

Synonyms

AGIF (Adipogenesis inhibitory factor)

Source

E. coli-derived

Molecular weight

Approximately 19.1kDa, a single non-glycosylated polypeptide chain containing179 amino acids.

Accession

P20809 Pro22-Leu199 with an N-terminal methionine

AA Sequence

MPGPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL

Purity

＞96% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of murine T11 cells is ≤ 2.0ng/ml, corresponding to a specific activity of ≥ 5 x 10⁵ units/mg.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB, 150mM NaCl, 150mM glycine,5% trehalose, pH7.2.

Endotoxin level

＜0.01EU/μg rHuIL-11 protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃.

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.