Recombinant Bovine Enterokinase Light Chain, Yeast
  • Recombinant Bovine Enterokinase Light Chain, Yeast

Recombinant Bovine Enterokinase Light Chain, Yeast

货      号 SB-ZR110
规      格 100U
品      牌 圣尔生物share-bio
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产品编号 名称 包装 品牌 目录价 促销价 货期 数量 购物车
SB-ZR110 Recombinant Bovine Enterokinase Light Chain, Yeast 100U 圣尔生物share-bio ¥480.00
SB-ZR110 Recombinant Bovine Enterokinase Light Chain, Yeast 1kU 圣尔生物share-bio ¥3200.00
SB-ZR110 Recombinant Bovine Enterokinase Light Chain, Yeast 250U 圣尔生物share-bio ¥1200.00

Recombinant Bovine Enterokinase Light Chain, Yeast 介绍

产品详情

Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which contains the catalytic subunit. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. Recombinant EK was reported to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. Recombinant bovine EK has higher activity than EK from other species and is used wildly in biochemical applications.

Product Information

Category

Recombinant protein

Synonyms

Enterokinase, Serine Protease 7, Transmembrane Protease Serine 15

Source

Expressed in Pichia Pastoris.

Molecular weight

Approximately 43.0kDa, a single glycosylated polypeptide chain containing 235 amino acids.

Protein amino acid sequence

IVGGSDSREGAWPWVVALYFDDQQVCGASLVSRDWLVSAAHCVYGRNMEPSKWKAVLGLHMASNLTSPQIETRLIDQIVINPHYNKRRKNNDIAMMHLEMKVNYTDYIQPICLPEENQVFPPGRICSIAGWGALIYQGSTADVLQEADVPLLSNEKCQQQMPEYNITENMVCAGYEAGGVDSCQGDSGGPLMCQENNRWLLAGVTSFGYQCALPNRPGVYARVPRFTEWIQSFLH

Unit Definition

One unit is defined as the amount of enzyme needed to cleave 50μg of fusion protein in 16 hours to 95% completion at 25 °C in a buffer containing 25mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2

Physical appearance

Sterile liquid

Endotoxin level

1EU/ug rBoEK protein

Formulation

50 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 50 % glycerol.

Storage/ Stability

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 6 months from date of receipt, -20 to -70 °C as supplied.
- 3 months, -20 to -70 °C under sterile conditions after opening.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.

 

储存条件及期限 冻干粉于-20℃到-80℃长期保存3年,溶液状态保存期3个月,避免反复冻融。
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Recombinant Bovine Enterokinase Light Chain, Yeast 说明书

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For research use only. Not intended for human and animal therapeutic or diagnostic use.

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