Recombinant human CCL20

Product Name

Recombinant Human Macrophage Inflammatory Protein-3 alpha/CCL20

 (rHuMIP-3α/CCL20)

Synonyms

LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine

Source

Expressed in E. coli. 

Molecular weight

Approximately 8.0kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

Accession

P78556  Ala27-Met96

AA Sequence

ASNFDCCLGYTDRILHPKFIVGFTRQLANEGCDINAIIFHTKKKLSVCANPKQTWVKYIVRLLSKKVKNM

Purity

＞97% by SDS-PAGE or HPLC

Formulation

Lyophilized from Lyophilized from a 0.2μm filtered solution in PBS，pH7.4.

Biological activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50ng/ml.

Endotoxin level

Less than 0.1EU/mg of rHuMIP-3α/CCL20 as determined by LAL method

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

For long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution.

12 months from date of receipt, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution.

3 months, -20 to -70℃ under sterile conditions after reconstitution.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.